More info
Assay range | 78-20,000 pg/mL |
Sensitivity | 70 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human C4 standard: 1 vial
2. One 96-well plate coated with Human C4 Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human C4 Ab (100x): 80 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Complement component 4 is a naturally glycosylated (6.9%) polypeptide functioning as a part of the classical and the lectin pathways of complement. It is synthesized as a single-chain precursor molecule which is proteolytically processed to the three-chain disulphide-linked structure with alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. After activation by C1s, C4 is processed to C4a and C4b. C4a anaphylatoxin is a mediator of local inflammation and induces smooth muscle contraction. C4b, the major activation product, is an essential subunit of the C3 and C5 convertases of the classical complement pathway. C4 deficiency is associated with systemic lupus erythematosus.