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Assay principle
This assay employs a standard sanwich enzyme-linked immunosorbent assay (ELISA) technology to detect Hepatitis B Virus Surface Antigen (HBsAg) in the samples. A monoclonal antibody specific to HBsAg was precoated in microplate wells. The HBsAg in the sample, if presents, will bind to the antibody immobilized on the wells and interacts with another anti- HBsAg Antibody conjugated with HRP to form an immunocomplex. Following incubation and washing procedures to remove unbound substances, this reaction is visualized by the addition of the chromogen tetramethylbenzidine (TMB). After stopping the reaction with sulfuric acid, the blue color turns yellow. What can be measured at this point is the amount of color intensity proportional to the amount of antibody captured in the wells, and to the sample.
Kit Components
1 | Microplate precoated with HBsAg Ab | 1x96 well |
2 | Negative Control | 1 ml X 1 vial |
3 | Positive Control | 1 ml X 1 vial |
4 | Diluent buffer | 13 mL x1 |
5 | Wash Solution (20x) | 50 mL x1 |
6 | Anti-HBsAg -HRP Conjugate | 7 ml x1 |
7 | Chromogenic Solution A | 8 ml |
8 | Chromogenic Solution B (TMB) | 8 ml |
9 | Stop Solution | 8 ml |