More info
Assay Range | 625-40,000 pg/mL |
Sensitivity | 600 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human C7 standard: 1 vial
2. One 96-well plate coated with Human C7 Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human C7 Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Human Complement Component 7 (C7) is an 821 amino acid (aa) single-chain glycoprotein with a molecular weight of about 92.4 KD. The N terminus of C7 shares 23-30% aa sequence identity with human Complement Components C8 and C9. C7 is essential for formation of the membrane attack complex (MAC) and is activated non-proteolytically by binding to recently-formed C5b, C6 complexes at the cell membrane. C7 is one of five complement proteins C5b, C6, C7, C8, and C9 that assemble on pathogen membranes to form the lethal membrane attack complex. It serves as a membrane anchor for the C5b-7 complex. C7 deficiency exhibits an increased susceptibility to Neisseria meningitides infections and recurrent meningococcal disease.