ELISA Kits

View larger

Human CD150/SLAM/SLAMF1 ELISA Kit

Catalog #: NS-E10189

Send to a friend

Send to a friend

Human CD150/SLAM/SLAMF1 ELISA Kit

Recipient :

Name of your friend ^* :

E-mail address of your friend ^* :

^* Required fields

or Cancel
Print

Data sheet

Assay Range	7.8 - 500 pg/ml

More info

Sensitivity

5 pg/mL

Size

96T

Storage

Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC

Assay Principle

Sandwich ELISA

Sample volume

100 µL final volume, dilution factor varies on samples.

Detection Method

Chromogenic

Kit Components

1. Recombinant human CD150 standard: 1 ng/vial - 2.

2. One 96-well plate precoated with anti-human CD150 Ab

3. Sample diluent buffer: 12 mL x 1

4. HRP conjugated anti-Human CD150 Ab: 1 vial, dilution 1:500.

5. Detection Antibody diluent buffer: 12 mL x 1

6. 20 x Wash Solution: 25 ml x 1.

7. TMB color developing agent A: 6 ml.

8. TMB color developing agent B: 6 ml.

9. TMB stop solution: 6ml.

Background

CD150/signaling lymphocytic activation molecule (SLAM) is a cell surface phosphoglycoprotein and belongs to the CD2 subset of the Ig superfamily of type I transmembrane glycoproteins. It is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. CD150 is an important high-affinity self-ligand in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. There are two SLAM signalings: one in which the inhibitor SH2D1A acts as a negative regulator and the other in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.

Standard Curve

Download

Protocol

Download (0)