More info
Assay Range | 31.2-2000 pg/mL |
Sensitivity | 5.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Sample Type | Cell culture supernatants. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Canine IFN-gamma standard: 2 vials
2. One 96-well plate coated with Canine IFN-gamma Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer:12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogenic solution A: 6 mlx1
9. Chromogenic solution B: 6 mlx1
10. Stop solution: 6 mL x1
11. Washing solution (20x): 25 mL x1
Background
Interferon gamma (IFN-γ), also known as type II interferon, is a member of the cytokine family and the only member of the type II class of interferons identified so far. IFN-γ is produced by a number of cell types under inflammatory conditions, including dendritic epidermal/γδ T cells, keratinocytes, peripheral blood γδ T cells, mast cells, neurons, CD8+ T cells, macrophages, B cells, neutrophils, NK cells, CD4+ T cells, and testicular spermatids. Rat IFN-γ shares approximately 87% and 39% amino acid sequence identity with mouse IFN-γ and human IFN-γ, respectively. Consistent with their degrees of shared homology, rat IFN-γ is active on mouse cells but not on human cells. Biologically active IFN-γ consists of a noncovalently linked homodimer which binds to transmembrane IFN-γ RI (alpha subunit) coupled with transmembrane IFN-γ RII (beta subunit) to form a functional receptor complex of two α and two β subunits. IFN-γ is essential to promote anti-viral, anti-proliferative, and immunoregulatory activities. On many cell types, IFN-γ induces the production of cytokines and upregulates the expression of various membrane proteins including class I and II MHC antigens, Fc receptors, leukocyte adhesion molecules, and B7 family antigens. IFN-γ also plays a key role in the pathogenesis of certain inflammatory diseases such as autoimmunity and atherosclerosis.