Canine CXCL8/IL-8 ELISA Kit

Kit Components

 1. Recombinant Human CANINE CXCL8   standard: 2 vials

 2. One 96-well plate coated with Human CANINE CXCL8   Ab

 3. Diluent buffer 1: 12 ml

 4. Diluent buffer 2 (4x): 20 ml

 5.Detection antibody: 12 ml

 6.Chromogenic Solution A : 11 ml.

 7. Chromogenic Solution B : 11 ml.

 8. Stop solution: 11 mL x1

 9. Washing solution (20x): 25 mL x1

Background

CXCL8, also known as Interleukin-8 (IL-8), GCP-1, and NAP-1, is a secreted protein belonging to the CXC chemokine family. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is the most bioactive form of CXCL8, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). It displays chemotactic activity for neutrophils and plays an important role in mediating innate immune system response. In addition, it also plays a role in promoting angiogenesis. CXCL8-mediated signaling pathways are transduced by two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. Clinically, CXCL8 may be related to the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.