More info
Assay Range | 25-800 ng/ml |
Sensitivity | 1.0 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Serum, plasma or other suitable solution. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Canine IgG standard: 2 vials
2. One 96-well plate coated with Canine IgG Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 1 vial
5. Antibody diluent buffer: 12 mL x1
6. Chromogenic solution A: 6 mlx1
7. Chromogenic solution B: 6 mlx1
8. Stop solution: 6 mL x1
9. Washing solution (20x): 25 mL x1
Assay principle
This Canine IgG enzyme linked immunosorbent assay kit applies a technique called quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an antibody specific for Canine IgG. Standards or samples are then added to the microtiter plate wells and Canine IgG if presents, will bind to the antibody on the wells. A standardized preparation of HRP-conjugated anti-Canine IgG antibody is added to each well to “sandwich†the Canine IgG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Canine IgG and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm.