View larger
Dog/Canine Granulocyte Colony Stimulating Factor G-CSF ELISA Kit
BG-CAN11162
Dog/Canine Granulocyte Colony Stimulating Factor ELISA Kit
Data sheet
| Assay time | 4.5 Hours |
| Validity | 6 months |
| Assay Range | 25 – 1600 pg/mL |
| Size | 96T |
| Assay principle | The Canine G-CSF ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Canine G-CSF specific antibody has been pre-coated onto 96-well plate. Canine G-CSF present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Canine G-CSF detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Canine G-CSF captured on plate. |