More info
Size | 96T |
Specificity | No cross-reaction with other related substances detected |
Storage | Store at 2 - 8ºC. Keep detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA/Qualitative Detection |
Sample Volume | 100 µL final volume |
Sample Type | Serum or plasma |
Detection Method | Chromogenic |
Kit Components
1. One 96-well plate pre-coated with Leptospira Patoc 1 antigen
2. Negative Control: 1 vial
3.Positive Control: 1 vial
4. Diluent buffer: 2x30 ml
5. Biotinylated-anti- bovine IgG Conjugate: 1 vial
6. Streptavidin-HRP: 1 vial
7. TMB developing agent: 11 mL x1
8. Stop solution: 11 mL x1
9. Washing solution (20x): 25 mL x1
Assay principle
This assay employs the enzyme-linked immunosorbent assay (ELISA) technology to detect Leptospira IgG in the samples. Purified Leptospira Patoc 1 antigen was precoated in microplate wells. The Leptospira IgG in the sample, if presents, will bind to the antigen immobilized on the wells and interacts with biotinalyted anti-Bovine IgG which will subsequently interacts with streptavidin-HRP conjugate to form an immunocomplex. Following incubation and washing procedures to remove unbound substances, this reaction is visualized by the addition of the chromogen tetramethylbenzidine (TMB). After stopping the reaction with sulfuric acid, the blue color turns yellow. What can be measured at this point is the amount of color intensity proportional to the amount of antibody captured in the wells, and to the sample.