More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat CD86 standard: 2 vials
2. One 96-well plate precoated with anti- Rat CD86 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
B7-2, also known as CD86, B70, T-lymphocyte activation antigen CD86, and ETC1, is a type I membrane protein belonging to the B7 family of the immunoglobulin superfamily. Mature rat B7-2 consists of a 222 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 19 aa transmembrane segment, and a 46 aa cytoplasmic tail. The ECD of rat B7-2 shares 59% and 81% aa sequence identity with human and mouse B7-2, respectively. B7-2 is highly expressed on activated antigen presenting cells (APC), such as B cells, dendritic cells, and monocytes, as well as on vascular endothelial cells. B7-2 and its homolog and partner B7-1 exhibit overlapping but distinct activities. B7-2 is expressed earlier than B7-1 following APC activation and both proteins bind with higher affinity to CTLA-4 than to CD28. B7-2 promotes the stabilization of CD28 in the immunological synapse, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the immunological synapse. The relative participation of B7-1 and B7-2 in T cell co-stimulation can also change the Th1/Th2 bias of the immune response. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and provides TCR-independent co-stimulation. In addition, B7-1 and B7-2 bind CTLA-4, which is up-regulated and recruited to the immunological synapse at the onset of T cell activation. CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function.