More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse Thrombomodulin standard: 2 vials
2. One 96-well plate precoated with anti- Mouse Thrombomodulin Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Thrombomodulin, also known as Fetomodulin, BDCA-3 and CD141, is a transmembrane protein encoded by the THBD gene in humans. Thrombomodulin is composed of an N-terminal C-type lectin domain, six contiguous epidermal growth factor (EGF)-like domains, a highly glycosylated serine/threonine-rich domain, a transmembrane segment, and a short C-terminal cytoplasmic tail. The full-length mouse Thrombomodulin is a 577 amino acid (aa) precursor protein containing a signal peptide and the mature polypeptide. Mature mouse Thrombomodulin is composed of a 501 aa extracellular domain (ECD) with a N-terminal C-type lectin domain and six EGF-like domains, a 24 aa transmembrane segment, and a 36 aa cytoplasmic domain. The ECD of mouse Thrombomodulin shares 66% and 85% aa sequence identity with human and rat counterparts, respectively. Thrombomodulin is expressed primarily by vascular endothelial cells. Thrombomodulin plays an important role in the anti-coagulation and fibrinolysis system. When the coagulation cascade is activated, prothrombin is converted to thrombin by coagulation factors Va and VIIIa, ultimately leading to fibrin clot formation. Thrombin binds to thrombomodulin and then activates protein C to degrade coagulation factors Va and VIIIa, thereby reducing the amount of thrombin generated and inhibiting coagulation. Thrombomodulin-thrombin complex also activates thrombin-activatable fibrinolysis inhibitor (TAFI), creating a carboxypeptidase that inhibits fibrinolysis. It has been shown that patients suffering from cardiovascular diseases, such as acute coronary syndrome, pulmonary thromboembolism, and severe hemorrhage, have increased circulating thrombomodulin concentrations.