More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse Prolactin standard: 2 vials
2. One 96-well plate precoated with anti- Mouse Prolactin Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Prolactin (PRL), also known as luteotropic hormone or luteotropin, is a secreted neuroendocrine pituitary hormone that acts primarily on the mammary gland to promote lactation. Human Prolactin is predominantly found as 199 amino acid, 25 kDa glycosylated and 23 kDa non-glycosylated monomers. In mice, however, the non-glycosylated monomeric form is the main source of Prolactin. Mouse prolactin shares 60% and 85% amino acid sequence identity with human and rat prolactin, respectively. Cleavage of prolactin by Cathepsin D, matrix metalloproteinases, or thrombin can produce N-terminal or C-terminal 16 kDa fragments with or without antiangiogenic activities. In humans, the large protein complex macroprolactin consisting of prolactin, IgG and prolactin autoantibodies contributes to high serum or plasma prolactin levels and shows decreased prolactin bioactivity. Prolactin is synthesized by the anterior pituitary in all mammals. In humans, prolactin is also produced by various cell types and tissues, while in rodents, the only known non-pituitary source is the placenta.
The prolactin receptor PRLR is a type I transmembrane glycoprotein belonging to the hematopoietic receptor family. PRLR is widely expressed in many different cells and tissues, including most leukocytes, CD34+ progenitor cells, mammary gland, liver, kidney, adrenals, ovaries, testis, prostate, seminal vesicles, and hypothalamus. In addition to its lactogenic activity, peripherally produced prolactin has exhibited diverse functions in regulation of reproduction, immunoregulation, as well as breast and prostate cancer development.