More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 2.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse RANK standard: 2 vials
2. One 96-well plate precoated with anti- Mouse RANK Ab
3. Sample diluent buffer: 12 ml— 2
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
RANK (receptor activator of NFκB), also known as TRANCE receptor, osteoclast differentiation factor receptor (ODFR) and TNFRSF11A, is a member of the tumor necrosis factor receptor family. The murine RANK is synthesized as a 316 amino acid (aa) type II transmembrane protein with a predicted 48 aa cytoplasmic domain and a 247 aa extracellular domain. The extracellular domain of mouse RANK shares 81% amino acid identity with its human counterpart. RANK is highly expressed in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
RANK is a receptor for TRANCE (TNF related activation induced cytokines, also known as RANK ligand, RANKL), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF). RANK plays an important role in osteoclast differentiation and activation, bone remodeling and repair, immune cell function, lymph node development, thermal regulation, as well as mammary gland development. RANKL/RANK triggers TRAF-6 -mediated activation of the c-jun N-terminal kinase (JNK) and nuclear factor kappa-b (NF-kB) pathways which promote differentiation and activation of osteoclasts. Osteoprotegerin (OPG) is a decoy receptor for RANK, and regulates the stimulation of the RANK signaling pathway by competing for RANKL.