More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse PECAM-1 standard: 2 vials.
2. One 96-well plate precoated with anti- Mouse PECAM-1 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Platelet endothelial cell adhesion molecule (PECAM-1), also known as cluster of differentiation 31 (CD31), EndoCAM, GPIIA', PECA1, is a protein encoded by the PECAM1 gene in humans. The extracellular domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig like domains, the first of which is critical for adhesion and extravasation. The cytoplasmic domain contains immunoregulatory tyrosine-based inhibitory and switch motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine mediated engagement of SH2 containing signaling molecules. PECAM-1 is normally found on endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T/NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils. PECAM-1 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. Mouse PECAM-1 ECD shows 77% and 63%amino acid (aa) identity with rat and human PECAM1, respectively. As a cell adhesion molecule, PECAM-1 is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It can prevent phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and change function on apoptosis to promote tethering of dying cells to phagocytes.