Human GH ELISA Kit

Kit Components

 1. Recombinant  Human Growth Hormone: 2 vials

 2. One 96-well plate coated with anti-Human Growth Hormone Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

Human growth hormone (GH or GH-N), also known as somatotropin, growth hormone 1, pituitary growth hormone, is a secreted protein encoded by the normal GH (GH-N/GH1) gene on humans. Human GH is synthesized as a 217 amino acid (aa) precursor protein with various forms generated by alternative splicing, proteolytic cleavage, post-translational modification, and association with binding proteins. The two most studied human GH forms include the full-length 22 kDa mature protein and a truncated 20 kDa form differing by the deletion of 32-46 aa residues.

GH is released into the circulation in pulsatile fashion and is regulated by the activities of several peptides and proteins in a manner that may vary between species. GH-releasing Hormone and the peptide hormone Ghrelin enhance human GH secretion, while Somatostatin suppresses secretion. Human GH exerts its biological activities via binding to the GH receptor (GHR), a type I cytokine receptor expressed by a wide variety of cell types. It is associated with several downstream signaling cascades that include JAK/STAT, PI3-Kinase, and MAP kinase. The growth-promoting effects of human GH may occur directly, or indirectly through stimulating the production of IGF-I. GH may also have an immunomodulatory role through its effects on the proliferation and activities of B cells, T cells, NK cells, macrophages, and neutrophils. In addition, GH can induce lipolysis, lactation, amino acid uptake, and protein synthesis.