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Human Ghrelin ELISA Kit
NB-E10638
Human growth hormone releasing peptide-Ghrelin,GHRP-Ghrelin ELISA Kit For research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA
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Human Ghrelin ELISA Kit
| Assay principle | The Human Ghrelin ELISA Kit is based on sandwich enzyme-linked immunosorbent assay technology. Anchor antibody against human Ghrelin specific antibody has been pre-coated onto 96-well plate. Human Ghrelin present in the standards/ samples bind to the capture antibody (anti-human Ghrelin IgG). Simultaneously, biotinylated anti-Human Ghrelin detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Human Ghrelin captured on plate. |
| Sample Type | Serum or plasma and other suitable sample solution |
| Assay Time | 4.5 Hours |
| Assay Range | 156 – 10,000 pg/mL |
| Sensitivity | 97.1 pg/mL |
| Specificity | No cross reactivity with relevant targets |
| Sample Level | Please check publications |
| Dilution Factor | 1/5 |
| Background | Ghrelin is a 28 amino acid multifunctional hormone produced primarily by the stomach with smaller amounts produced in the placenta, kidney, pituitary and hypothalamus. Ghrelin is synthesized as a preprohormone, and then proteolytically processed to yield a 28-amino acid peptide. N-octanoylation of serine3 position in the molecule is necessary for its bioactivity. Originally found as an endogenous ligand for the growth hormone secretagogue receptor in the pituitary gland, it distinguishes itself from the hypothalamic growth hormone-releasing hormone as another potent stimulator for growth hormone secretion. It is also an important orexigenic hormone in the regulation of energy homeostasis. Ghrelin increases food intake and thus fat mass by an action exerted at the level of the hypothalamus. They activate cells in the arcuate nucleus that include the orexigenic neuropeptide Y (NPY) neurons. Ghrelin-responsiveness of these neurons is both leptin and insulin sensitive. Ghrelin also activates the mesolimbic cholinergic-dopaminergic reward link, a circuit that communicates the hedonic and reinforcing aspects of natural rewards, such as food. |
Different lot may vary, please follow the instruction coming with the kit.