View larger
More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 15.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human sCD40L standard: 2 vials
2. One 96-well plate precoated with anti-Human sCD40L Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
CD40 Ligand (CD40L), also known as CD154, gp39, TNFSF5 (tumor necrosis factor ligand superfamily member 5), TRAP (TNF-Related Activation Protein) or TBAM (T-cell B-cell Activating Molecule), is a multifunctional ligand in the TNF superfamily. Natural CD40L occurs as a 39 kDa, 261 amino acid (aa) type II transmembrane glycoprotein that can form homotrimers or as a 15-18 kDa soluble form (sCD40L) produced by proteolytic cleavage. sCD40L lacks the transmembrane region and a portion of the extracellular domain but contains the entire TNF- homologous region. Both of these two forms of CD40L are expressed on activated T cells and platelets and show biological activities.
CD40L is expressed primarily on activated CD4+ T cells, although many other cells including vascular endothelial cells, smooth muscle cells, macrophages, basophils, eosinophils, monocytes, dendritic cells, fibroblasts, and mast cells also express CD40L. The receptor for CD40L is CD40/ TNFRSF5, a member of the TNF receptor superfamily. Interaction of CD40L with CD40 induces proliferation and isotype switching in B lymphocytes and mediates a broad variety of other immune and inflammatory responses. Interruption of CD40 signaling transduction can lead to impairment of T lymphocyte function, B lymphocyte differentiation, as well as monocyte function and has been implicated in the pathogenesis of certain chronic inflammatory diseases such as autoimmune diseases, neurodegenerative disorders, graft-versus-host disease, cancer, and atherosclerosis.