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Hepatitis B Virus Core delta Recombinant ( HBV core delta )
DescriptionThe E.coli derived 14kDa recombinant protein contains the HBV core delta ayw immunodominant region amino acids 1-144.Purification MethodPurified by proprietary chromatographic technique.PurityHBV Core Delta protein is >95% pure as
Data sheet
| Storage | HBV Core Delta protein although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Formulation | 7.5mM Phosphate buffer pH-7.2, 75mM NaCl, & 50% glycerol. |
| Purity | HBV Core Delta protein is >95% pure as determined by SDS-PAGE (coomassie staining). |
| Applications | HBV Core Delta antigen is suitable for ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems. |
| Description | The E.coli derived 14kDa recombinant protein contains the HBV core delta ayw immunodominant region amino acids 1-144. |
| Protein Background | Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA. |
| Purification Method | Purified by proprietary chromatographic technique. |
| Human Virus Test | The Iron content was estimated by ICP and was found to be 1228 ppm. |
| Advantages | FDA approved Plasma from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG, HBc, ALT and Syphilis.Viral inactivation by pasteurization (60°C for 10 hours) has been validated using three different test viruses, with removal of 8-14.5 logs of virus documented. The purification process has also been found to remove significant additional quantities of virus. |