More info
Assay range | 3.125 -200 µg/ml |
Sensitivity | 3.1 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Competitive ELISA |
Sample Volume | 25 µL final volume, dilution factor varies on samples |
Sample Type | serum, plasma and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human LDL standard: 1 vial
2. One 96-well plate coated with Human LDL Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human LDL: 1 vial
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7 . Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Lipoproteins are complex particles composed of multiple proteins which transport all fat molecules (lipids) in the body. Lipoproteins can be classified into chylomicrons (ULDL), very low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein (LDL), and high-density lipoprotein (HDL). LDL consists of a neutral lipid core surrounded by an outer shell of phospholipids and amphipathic apolipoprotein B-100, a ligand for hepatic clearance of plasma cholesterol via LDL receptors (LDL R). LDL exhibits its activity in transporting cholesterol from the liver to tissues where it is incorporated into cell membranes. It has been shown that elevation of small, dense LDL in plasma is associated with increased risk of myocardial infarction. Familial hypercholesterolemia caused by a mutation in an LDL receptor gene is associated with elevated LDL levels and premature coronary atherosclerosis.