More info
Assay range | 1.56-50 ng/ml |
Sensitivity | 1.5 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Serum, plasma or cell supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Glucokinase standard: 1 vial
2. One 96-well plate coated withHuman Glucokinase Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human Glucokinase Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Glucose kinase (GCK), also known as hexokinase IV, is a member of mammalian hexokinases which contain four isoforms (I, II, III and IV). Unlike other forms of hexokinases, which have high affinity for glucose and are strongly inhibited by the product, glucose-6-phosphate, GCK has much lower affinity for glucose and is not inhibited by glucose-6-phosphate. Consequently, GCK has a much higher Km for glucose than that of other hexokinases. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells. In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non-insulin dependent diabetes mellitus, maturity-onset diabetes of the young type 2, and hyperinsulinemia of infancy.