More info
Assay range | 31.2 to 2,000 ng/ml |
Sensitivity | 30.0 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Serum, plasma, CSF, or cell lysates/supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Apo-E standard: 1 vial
2. One 96-well plate coated with Human Apo-E Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human Apo-E Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Apolipoprotein E (ApoE) is a major protein component of serum LDL, VLDL, HDL, and chylomicrons. It is produced predominantly by hepatocytes, macrophages in peripheral tissues, and nonneuronal cells in the central nervous system. Mature human ApoE is a 34 kDa glycoprotein that consists of an N-terminal domain of four bundled α-helices, a hinge region, and an extended α-helical C-terminal domain. There are three common isoforms of this protein: apoE-3, apoE-2 and apoE-4. Mature human ApoE shares 71% amino acid (aa) sequence identity with mouse and rat ApoE.
Most LDL receptor family proteins preferentially bind and internalize the lipid-bound form of ApoE, while VLDLR internalizes lipid-free ApoE. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents and and also plays a critical role in amyloid metabolism and the development of Alzheimer’s disease. Differences in the effects of ApoE isoforms on cholesterol and amyloid Aβ peptide metabolism have been associated with metabolic syndrome, type II diabetes, atherosclerosis, and Alzheimer’s disease.