More info
Assay Range | 1.875-120 ng/ml |
Sensitivity | 1.8 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Factor D standard: 1 vial
2. One 96-well plate coated with Human Factor D Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human Factor D Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Complement factor D, also known as adipsin, is a serine protease that is required for the initiation of the alternative pathway of complement. Human complement factor D is a 253 amino acid (aa) precursor protein consisting of a signal peptide, a five-residue activation-/ pro-peptide, and the mature chain. Under physiological conditions, mature factor D lacking the activation peptide circulates as an inactive enzyme and requires interaction with its natural substrate, C3b-bound factor B, for activation of its catalytic activity. Active factor D functions to cleave the C3b-bound factor B, resulting in the formation of C3bBb complex, the alternative pathway C3 convertase. Factor D is expressed in multiple tissues, including monocyte/ macrophages, muscle, sciatic nerve, endometrium, kidney, intestine, and at especially high levels in adipocytes. Pathologically, Complement factor D deficiency is associated with low activity of the alternative complement pathway and low capacity to opsonize bacteria.