More info
Assay Range | 125-8,000 pg/mL |
Sensitivity | 120 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human C1r standard: 1 vial
2. One 96-well plate coated with Human C1r Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human C1r Ab (100x): 80 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
C1r enzyme is the activated form of C1r proenzyme and is a subunit of the C1 complex which is the first complement component in the classical pathway of complement. C1 is actually a non-covalent assembly of three different proteins (C1q, C1r, and C1s) bound together in a calcium-dependent complex. C1r precursor consists of a 17 amino acid (aa) signal peptide and a 688 aa mature protein. Upon C1q binding to the surface of pathogens, the activated C1r is cleavage into two chains, A and B, connected by disulfide bonds. The 446 aa non-catalytic amino-terminal C1r A chain contains a growth factor domain and two internal repeats. The 242 aa catalytic C1r B chain (light) is homologous to the trypsin family of serine proteases.
C1r plays a key role in mediation of innate immunity. The activated C1r is able to activate C1s which in turn activates C2 and C4, leading to the formation of the membrane attack complex and the elimination of the target. C1r deficiency is associated with autoimmune diseases such as systemic lupus erythematosus.