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Telomerase Antibody

ASA-B1843

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
telomerase reverse transcriptase Polyclonal IgG Rabbit Mouse, Rat WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Telomerase(15-30aa RSRYREVWPLATFVRR), identical to the related rat sequence.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 TERT

Protein

 Telomerase reverse transcriptase

Uniprot ID

 O70372

Function

 Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA- dependent extension of 3'-chromosomal termini with the 6- nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis (By similarity).

Tissue Specificity

High activity in intestine, liver and testis, moderate in lung, very low in muscle, heart and brain.

Sub-cellular localization

 Nucleus, nucleolus . Nucleus, nucleoplasm . Nucleus. Chromosome, telomere. Cytoplasm . Nucleus, PML body . Note: Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress- mediated phosphorylation at Tyr-697. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT (By similarity).

Sequence Similarities

 Belongs to the reverse transcriptase family. Telomerase subfamily.

Aliases

 EST2 antibody|hEST2 antibody|htert antibody|TCS1 antibody|Telomerase associated protein 2 antibody|Telomerase Catalytic Subunit antibody|Telomerase reverse transcriptase antibody|Telomerase-associated protein 2 antibody|Telomere Reverse Transcriptase antibody|TERT antibody|TERT_HUMAN antibody|TP2 antibody|TRT antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Mouse, RatAssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Telomerase antibody, ASA-B1843, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
Lane 4: Mouse Testis Tissue Lysate
Lane 5: Mouse Kidney Tissue Lysate
Lane 6: Mouse Heart Tissue Lysate

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