More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| SRY(sex determining region Y)-box 7 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SOX7(370-388aa LISVLADATATYYNSYSVS), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SOX7 |
Protein | Transcription factor SOX-7 |
Uniprot ID | Q9BT81 |
Function | Binds to and activates the CDH5 promoter, hence plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium and blocks further differentiation into blood precursors (By similarity). May be required for the survival of both hematopoietic and endothelial precursors during specification (By similarity). Competes with GATA4 for binding and activation of the FGF3 promoter (By similarity). Represses Wnt/beta-catenin-stimulated transcription, probably by targeting CTNNB1 to proteasomal degradation. Binds the DNA sequence 5'- AACAAT-3'. |
Tissue Specificity | Widely expressed in adult and fetal tissues. Present both in mesenchymal and epithelial cells in some adult tissues, including colon. Tends to be down-regulated in prostate adenocarcinomas and colorectal tumors due to promoter hypermethylation. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 1 HMG box DNA-binding domain. |
Aliases | MGC10895 antibody|SOX 7 antibody|SOX 7 transcription factor antibody|sox7 antibody|SOX7 transcription factor antibody|SRY(sex determining region Y) box 7 antibody|SRY box 7 antibody||SRY sex determining region Y box 7 antibody||Transcription factor SOX 7 antibody|Transcription factor Sox-7 antibody|Transcription factor SOX7 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SOX7 antibody, ASA-B1762, All Western blotting
All lanes: Anti-SOX7(ASA-B1762) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 40ug
Lane 3: Rat Lung Tissue Lysate at 40ug
Lane 4: Rat Testis Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: HEPG2 Whole Cell Lysate at 40ug
Lane 8: SMMC Whole Cell Lysate at 40ug
Lane 9: NEURO Whole Cell Lysate at 40ug
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All lanes: Anti-SOX7(ASA-B1762) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 40ug
Lane 3: Rat Lung Tissue Lysate at 40ug
Lane 4: Rat Testis Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: HEPG2 Whole Cell Lysate at 40ug
Lane 8: SMMC Whole Cell Lysate at 40ug
Lane 9: NEURO Whole Cell Lysate at 40ug