More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nitric oxide synthase 2, inducible | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human NOS2(3-24aa CPWKFLFKTKFHQYAMNGEKDI), different from the related rat sequences by nine amino acids, and from the related mouse sequences by eight amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NOS2 |
Protein | Nitric oxide synthase, inducible |
Uniprot ID | P35228 |
Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. As component of the iNOS- S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys- 247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. |
Tissue Specificity | Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the NOS family. |
Aliases | HEP NOS antibody|HEP-NOS antibody|Hepatocyte NOS antibody|HEPNOS antibody|inducible antibody|Inducible nitric oxide synthase antibody|Inducible NO synthase antibody|Inducible NOS antibody|INOS antibody|Inosl antibody|MAC NOS antibody|Macrophage NOS antibody|Nitric oxide synthase 2 inducible antibody|Nitric oxide synthase 2 inducible macrophage antibody|Nitric oxide synthase 2A(inducible hepatocytes) antibody|Nitric oxide synthase antibody|Nitric oxide synthase inducible antibody|NOS 2 antibody|NOS 2A antibody|NOS antibody|Nos II antibody|NOS type II antibody|Nos2 antibody|NOS2_HUMAN antibody|NOS2A antibody|Peptidyl-cysteine S-nitrosylase NOS2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- -NOS2 antibody, ASA-B1397, Western blotting
All lanes: Anti-NOS2 antibody at0.5ug/ml, ASA-B1397
Lane 1: Rat brain tissue Lysate at 50ug
Lane 2: Mouse brain tissue Lysate at 50ug
Lane 3: Rat PC12 whole Cell Lysate at 40ug
Lane 4: Rat NRK whole Cell Lysate at 40ug
Lane 5: Hela whole Cell Lysate at 40ug
Lane 6: Human placenta tissue Lysate at 50ug
Lane 7: HT1080 whole Cell Lysate at 40ug
Lane 8: MM1231 whole Cell Lysate at 40ug
Predicted bind size:
All lanes: Anti-NOS2 antibody at0.5ug/ml, ASA-B1397
Lane 1: Rat brain tissue Lysate at 50ug
Lane 2: Mouse brain tissue Lysate at 50ug
Lane 3: Rat PC12 whole Cell Lysate at 40ug
Lane 4: Rat NRK whole Cell Lysate at 40ug
Lane 5: Hela whole Cell Lysate at 40ug
Lane 6: Human placenta tissue Lysate at 50ug
Lane 7: HT1080 whole Cell Lysate at 40ug
Lane 8: MM1231 whole Cell Lysate at 40ug
Predicted bind size:
