MUC4 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
mucin 4, cell surface associated	Polyclonal	IgG	Rabbit	Human, Rat	WB	Immunogen affinity purified.
Immunogen
E.coli-derived human MUC4 recombinant protein (Position: M1249-D1444). Human MUC4 shares 69% and 36% amino acid (aa) sequences identity with mouse and rat MUC4, respectively.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	MUC4
Protein	Mucin-4
Uniprot ID	Q99102
Function	May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.
Tissue Specificity	Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines.
Sub-cellular localization	Membrane ; Single-pass membrane protein . Secreted.
Sequence Similarities	Contains 1 AMOP domain.
Aliases	Ascites sialoglycoprotein 1 antibody|Ascites sialoglycoprotein 2 antibody|Ascites sialoglycoprotein antibody|ASGP antibody|ASGP-1 antibody|ASGP-2 antibody|HSA276359 antibody|MUC-4 antibody|Muc4 antibody|MUC4_HUMAN antibody|Mucin 4 antibody|Mucin 4 cell surface associated antibody|Mucin 4 tracheobronchial antibody|Mucin-4 beta chain antibody|Pancreatic adenocarcinoma mucin antibody|Testis mucin antibody|Tracheobronchial mucin antibody|Tracheobronchial mucin Fragment antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Rat Human	AssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information