More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mitofusin 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1 (23-49aa DQLLEFVTEGSHFVEATYKNPELDRIA), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MFN1 |
Protein | Mitofusin-1 |
Uniprot ID | Q8IWA4 |
Function | Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. |
Tissue Specificity | Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers. |
Sub-cellular localization | Mitochondrion outer membrane. |
Sequence Similarities | Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily. |
Aliases | Fzo homolog antibody|Hfzo1 antibody|Hfzo2 antibody|MFN 1 antibody|Mfn1 antibody|MFN1_HUMAN antibody|Mitochondrial transmembrane GTPase Fzo 1 antibody|Mitochondrial transmembrane GTPase FZO 2 antibody|Mitochondrial transmembrane GTPaseFZO1B antibody|Mitofusin 1 antibody|Mitofusin-1 antibody|Mitofusin1 antibody|Putative transmembrane GTPase antibody|Transmembrane GTPase MFN1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Mitofusin 1 antibody, ASA-B1280, Western blotting
All lanes: Anti Mitofusin 1 (ASA-B1280) at 0.5ug/ml
WB: Recombinant Human Mitofusin 1 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti Mitofusin 1 (ASA-B1280) at 0.5ug/ml
WB: Recombinant Human Mitofusin 1 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- Mitofusin 1 antibody, ASA-B1280, Western blotting
All lanes: Anti Mitofusin 1 (ASA-B1280) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouset Cardiac Muscle Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Predicted bind size: 84KD
Observed bind size: 84KD
All lanes: Anti Mitofusin 1 (ASA-B1280) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouset Cardiac Muscle Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Predicted bind size: 84KD
Observed bind size: 84KD
