More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| GATA binding protein 3 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human GATA3 recombinant protein (Position: M1-E197). Human GATA3 shares 95% amino acid (aa) sequence identity with mouse GATA3. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GATA3 |
Protein | Trans-acting T-cell-specific transcription factor GATA-3 |
Uniprot ID | P23771 |
Function | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. |
Tissue Specificity | T-cells and endothelial cells. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 2 GATA-type zinc fingers. |
Aliases | GATA 3 antibody|GATA binding factor 3 antibody|GATA binding protein 3 antibody|GATA-binding factor 3 antibody|Gata3 antibody|GATA3_HUMAN antibody|HDR antibody|HDRS antibody|MGC2346 antibody|MGC5199 antibody|MGC5445 antibody|Trans acting T cell specific transcription factor GATA 3 antibody|Trans-acting T-cell-specific transcription factor GATA-3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GATA3 antibody, ASA-B0768, Western blotting
All lanes: Anti GATA3 (ASA-B0768) at 0.5ug/ml
WB: Recombinant Human GATA3 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti GATA3 (ASA-B0768) at 0.5ug/ml
WB: Recombinant Human GATA3 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- GATA3 antibody, ASA-B0768, Western blotting
All lanes: Anti GATA3 (ASA-B0768) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
All lanes: Anti GATA3 (ASA-B0768) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
