More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| fibroblast growth factor 21 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human FGF21(43-58aa QVRQRYLYTDDAQQTE), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FGF21 |
Protein | Fibroblast growth factor 21(FGF-21) |
Uniprot ID | Q9NSA1 |
Function | Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. |
Tissue Specificity | |
Sub-cellular localization | Secreted . |
Sequence Similarities | Belongs to the heparin-binding growth factors family. |
Aliases | FGF 21 antibody|FGF-21 antibody|Fgf21 antibody|FGF21_HUMAN antibody|FGFL antibody|Fibroblast growth factor 21 antibody|PRO10196 antibody|UNQ3115 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FGF21 antibody, ASA-B0701, Western blotting
All lanes: Anti FGF21 (ASA-B0701) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: A431 Whole Cell Lysate at 40ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
All lanes: Anti FGF21 (ASA-B0701) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: A431 Whole Cell Lysate at 40ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
