More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| embryonic ectoderm development | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human EED recombinant protein (Position: M256-R441). Human EED shares 100% amino acid (aa) sequence identity with mouse EED. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EED |
Protein | Polycomb protein EED |
Uniprot ID | O75530 |
Function | Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. |
Tissue Specificity | Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer. |
Sub-cellular localization | Nucleus. Chromosome. Note: Transiently colocalizes with XIST at inactive X chromosomes. |
Sequence Similarities | Belongs to the WD repeat ESC family. |
Aliases | eed antibody|EED protein antibody|EED, mouse, homolog of antibody|EED_HUMAN antibody|Embryonic ectoderm development antibody| Embryonic ectoderm development isoform a antibody|Embryonic ECTODERM development protein homolog antibody|Embryonic ectoderm development protein, mouse, homolog of antibody|HEED antibody|OTTHUMP00000235483 antibody|OTTHUMP00000235484 antibody| OTTHUMP00000235485 antibody|Polycomb protein eed antibody|WAIT 1 antibody|WAIT-1 antibody|WAIT1 antibody|WD protein associating with integrin cytoplasmic tails 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- EED antibody, ASA-B0634, Western blotting
All lanes: Anti EED (ASA-B0634) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 72KD
All lanes: Anti EED (ASA-B0634) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 72KD
