More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| DEAD(Asp-Glu-Ala-Asp) box helicase 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human DDX6(440-453aa YDDRFNLKSIEEQL), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DDX6 |
Protein | Probable ATP-dependent RNA helicase DDX6 |
Uniprot ID | P26196 |
Function | In the process of mRNA degradation, may play a role in mRNA decapping. |
Tissue Specificity | Abundantly expressed in most tissues. |
Sub-cellular localization | Cytoplasm, P-body. |
Sequence Similarities | Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. |
Aliases | ATP dependent RNA helicase DDX6 antibody|ATP dependent RNA helicase p54 antibody|DDX6 antibody|DEAD(Asp Glu Ala Asp) box polypeptide 6 antibody|DEAD box 6 antibody|DEAD box protein 6 antibody|DEAD/H(Asp Glu Ala Asp/His) box polypeptide 6(RNA helicase 54kD) antibody|FLJ36338 antibody|HLR2 antibody|Oncogene RCK antibody|P54 antibody|Probable ATP-dependent RNA helicase DDX6 antibody|RCK antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DDX6 antibody, ASA-B0582, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human DDX6, 41.8KD(162aa tag+S330-P483)
Lane 1: Recombinant Human DDX6 Protein 10ng
Lane 2: Recombinant Human DDX6 Protein 5ng
Lane 3: Recombinant Human DDX6 Protein 2.5ng
Lane 4: Recombinant Human DDX6 Protein 1.25ng
Recombinant Protein Detection Source: E.coli derived -recombinant human DDX6, 41.8KD(162aa tag+S330-P483)
Lane 1: Recombinant Human DDX6 Protein 10ng
Lane 2: Recombinant Human DDX6 Protein 5ng
Lane 3: Recombinant Human DDX6 Protein 2.5ng
Lane 4: Recombinant Human DDX6 Protein 1.25ng
