Keap1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
kelch-like ECH-associated protein 1	Polyclonal	IgG	Rabbit	Human	WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Keap1(46-60aa QHGNRTFSYTLEDHT), different from the related rat and mouse sequences by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	KEAP1
Protein	Kelch-like ECH-associated protein 1
Uniprot ID	Q14145
Function	Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
Tissue Specificity	Broadly expressed, with highest levels in skeletal muscle.
Sub-cellular localization	Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus.
Sequence Similarities	Contains 1 BACK (BTB/Kelch associated) domain.
Aliases	Cytosolic inhibitor of Nrf2 antibody|INrf 2 antibody|INrf2 antibody|Keap 1 antibody|KEAP1 antibody|KEAP1_HUMAN antibody|Kelch like ECH associated protein 1 antibody|Kelch like family member 19 antibody|Kelch like protein 19 antibody|Kelch-like ECH-associated protein 1 antibody|Kelch-like protein 19 antibody|KIAA0132 antibody|KLHL 19 antibody|KLHL19 antibody|MGC10630 antibody|MGC1114 antibody|MGC20887 antibody|MGC4407 antibody|MGC9454 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information