More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nuclear receptor coactivator 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human KAT13A recombinant protein (Position: H614-Q826). Human KAT13A shares 92% amino acid (aa) sequence identity with mouse KAT13A. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NCOA1 |
Protein | Nuclear receptor coactivator 1 |
Uniprot ID | Q15788 |
Function | Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3. |
Tissue Specificity | Widely expressed. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the SRC/p160 nuclear receptor coactivator family. |
Aliases | ASV antibody|bHLHe74 antibody|c SRC antibody|Class E basic helix-loop-helix protein 74 antibody|F SRC 1 antibody|Hin 2 protein antibody|Hin2 protein antibody|MGC129719 antibody|MGC129720 antibody|mNRC 1 antibody|NCoA 1 antibody|NCoA-1 antibody|Ncoa1 antibody|NCOA1_HUMAN antibody|Nuclear receptor coactivator 1 antibody|Nuclear receptor coactivator protein 1 antibody|NY REN 52 antigen antibody|p60 Src antibody|pp60c src antibody|Protein Hin 2 antibody|Protein Hin-2 antibody|Protein Hin2 antibody|Proto oncogene tyrosine protein kinase Src antibody|Renal carcinoma antigen NY REN 52 antibody|Renal carcinoma antigen NY-REN-52 antibody|RIP 160 antibody|RIP160 antibody|SRC 1 antibody|SRC-1 antibody|Steroid receptor coactivator 1 antibody|v src avian sarcoma (Schmidt Ruppin A 2) viral oncogene homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- KAT13A antibody, ASA-B1101, Western blotting
All lanes: Anti KAT13A (ASA-B1101) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
All lanes: Anti KAT13A (ASA-B1101) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
