More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| integrin, alpha 5 (fibronectin receptor, alpha polypeptide) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Integrin alpha 5 recombinant protein (Position: E787-E992). Human Integrin alpha 5 shares 86% amino acid (aa) sequence identity with mouse Integrin alpha 5. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ITGA5 |
Protein | Integrin alpha-5 |
Uniprot ID | P08648 |
Function | Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. |
Tissue Specificity | |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the integrin alpha chain family. |
Aliases | CD49 antigen-like family member E antibody|CD49e antibody|Fibronectin Receptor alpha antibody|Fibronectin receptor subunit alpha antibody|Fibronectin receptor, alpha subunit antibody|FNRA antibody|Integrin alpha 5 (fibronectin receptor alpha) antibody|Integrin alpha-5 antibody|Integrin alpha-5 light chain antibody|Integrin alpha-F antibody|Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody|ITA5_HUMAN antibody|ITGA 5 antibody|ITGA5 antibody|Very Late Activation Protein 5 alpha antibody|Very late activation protein 5, alpha subunit antibody|VLA-5 antibody|VLA5 antibody|VLA5A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Integrin alpha 5 antibody, ASA-B1044, Western blotting
All lanes: Anti Integrin alpha 5 (ASA-B1044) at 0.5ug/ml
WB: Recombinant Human Integrin alpha 5 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti Integrin alpha 5 (ASA-B1044) at 0.5ug/ml
WB: Recombinant Human Integrin alpha 5 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- Integrin alpha 5 antibody, ASA-B1044, Western blotting
All lanes: Anti Integrin alpha 5 (ASA-B1044) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: 293T Whole Cell Lysate at 40ug
Lane 7: NEURO Whole Cell Lysate at 40ug
Lane 8: PC-12 Whole Cell Lysate at 40ug
Predicted b
All lanes: Anti Integrin alpha 5 (ASA-B1044) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: 293T Whole Cell Lysate at 40ug
Lane 7: NEURO Whole Cell Lysate at 40ug
Lane 8: PC-12 Whole Cell Lysate at 40ug
Predicted b
