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Centromere Protein B Human Recombinant ( CENPB Human )
DescriptionCENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 65,940 Dalton. CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.SourceSf9
Data sheet
| Formulation | CENPB is supplied at a concentration of in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Applications | Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera. |
| Description | CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kDa. CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques. |
| Protein Background | Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.CENPB is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENPB antibodies are present in the sera of up to 80% of patients with CREST syndrome. |
| Expression host | Sf9 insect cells. |
| Synonyms | Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB. |
| Reagent Appearance | Sterile Filtered liquid formulation. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Immunological functions | 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera). |
| Coating concentration | 0.15-0.375 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination. |