Ku P70/P80 Human Recombinant ( Ku P70/P80 Human )

Data sheet

Formulation

KuP70/P80is supplied in 16mM HEPES buffer pH-8, 160mM NaCl, and 20% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Applications

Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera.

Description

KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit and 83,528 Dalton for the p80 subunit. Ku (P70/P80) is expressed with a -6xHis tag and purified by p

Protein Background

Ku antigen consists of two polypeptides (70 kDa and 80 kDa; the p80 subunit is occasionally called p86). The Ku heterodimer provides DNA-binding and regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination proce

Expression host

Sf9 insect cells.

Synonyms

ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair pr

Reagent Appearance

Sterile Filtered clear solution.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Coating concentration

0.2-0.6 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.