Small Nuclear Ribonucleoprotein 70kDa Human Recombinant ( SNRNP70 Human )

Data sheet

Formulation

U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA and 0.02% SDS.

Purity

Greater than 90% as determined by SDS-PAGE.

Applications

Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera (Mixed Connective Tissue Disease).

Description

Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein (lacking 66 internal amino acids outside the known epitope-containing areas) fused to a hexahistidine purification tag.The Calculated molecular weight is 44

Protein Background

Small nuclear ribonucleoprotein complexes (abbreviated as U-snRNP) are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with sever

Expression host

Escherichia Coli.

Synonyms

U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP.

Reagent Appearance

Sterile Filtered clear solution.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Coating concentration

0.3-0.6 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.