More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat Lipocalin-2 standard: 2 vials.
2. One 96-well plate precoated with anti- Rat Lipocalin-2 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Pentraxin 3 (PTX3), also known as Pentaxin-related protein PTX3, Tumor necrosis factor alpha-induced protein 5 (TNF alpha- induced protein 5), Tumor necrosis factor-inducible gene 14 protein (TSG-14) , is a large pentraxin belonging to the pentraxin superfamily characterized by cyclic multimeric structure. Pentraxins are multi-functional pattern-recognition proteins that have a characteristic pentagonal discoid arrangement. PTX3 forms decamers and higher-order multimers through the use of interchain disulfide bonds. The secreted mouse PTX3 is a glycoprotein with a 162 amino acid (aa) N-terminal extension and a 202 aa C-terminal pentraxin domain. PTX3 is secreted by a variety of cell types including macrophages, neutrophils, myeloid-derived dendritic cells, ovarian granulosa cells, endothelial cells, fibroblasts, adipocytes, renal mesangial cells, synovial cells, smooth muscle cells, alveolar epithelium, and glial cells and it is induced in response to pro-inflammatory stimuli and Toll-like Receptor (TLR) engagement. In humans and mice, PTX3 is an acute phase protein and increases rapidly in plasma during inflammatory and infectious conditions. PTX3 binds with high affinity to the complement component C1q, the extracellular matrix component TNFα induced protein 6 (TNFAIP6, TNF-stimulated gene 6, TSG-6) and selected microorganisms. Interaction of PTX3 with its soluble ligands plays an important role in several physiological conditions, ranging from innate immunity to female fertility. PTX3 has a dual role in the regulation of the innate immune response. Through its C-terminal pentraxin domain, immobilized PTX3 will bind complement component C1q to induce classical complement activation. Conversely, fluid-phase PTX3 inhibits classical complement activation. While PTX3 enhances C1q binding on apoptotic cells and facilitates the complement-mediated clearance of these cells, it can also bind apoptotic cells and inhibit their uptake by dendritic cells, preventing the onset of an autoimmune response. PTX3 interacts with selected viral, fungal, and bacterial components, providing protection from infection and may act as an opsonin. Through its interaction with TSG-6, an extracellular matrix hyaluronan (HA)-binding protein, PTX3 is involved in the assembly of the HA-rich extracellular matrix of the cumulus oophorus, which is essential for female fertility.