More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat TrkA standard: 2 vials.
2. One 96-well plate precoated with anti- Rat TrkA Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Tyrosine kinase receptor A (TrKA), also known as neurotrophic tyrosine kinase receptor type 1 or TRK1-transforming tyrosine kinase protein, is a member of the neurotrophic tyrosine kinase receptor family consisting of three members, Trk A, Trk B and Trk C. The Trk proteins share a common structure of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, two immunoglobulin-like domains in the extracellular region, as well as an intracellular region with the tyrosine kinase domain. Nerve growth factor (NGF) interacts with the cell surface receptor complex composed of TrKA and p75 to promote survival and differentiation of neurons. TrkA is a high affinity receptor for NGF. Binding of NGF to TrKA leads to homodimerization and autophosphorylation of TrKA which recruits, phosphorylates and activates a series of downstream molecules including SHC1, FRS2, SH2B1, SH2B2 and PLCG1. SHC1 and FRS2 activate a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Activation of PLCG1 controls NF-Kappa-B regulated transcription of genes involved in cell survival. Activation of SHC1 and SH2B1 triggers the Ras-PI3 kinase-AKT1 signaling pathway to regulate cell survival. TrKA can also bind NT-3. However, interaction of NT-3 with TrKA only supports axonal extension but has no effect on neuron survival.