Data sheet
| Specificity | No detectable cross-reactivity with any other cytokine. |
More info
Product name
Rat IL-6 ELISA Kit
Catalog No.
FM-E100068
Size
96T
Range
62.5pg/mL-4000pg/mL
Sensitivity
Less Than 5pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
serum, body fluids, tissue lysates or cell culture supernates.
Kit Components
1. Lyophilized recombinant rat IL-6 standard: 10ng/tube×2.
2. One 96-well plate precoated with anti- rat IL-6 antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- rat IL-6 antibody : 130μl, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130μl, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH). Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.
Rat IL-6 ELISA Kit-1X96 Well Plate Image