| Formulation |
PSIP1 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
| Purity |
Greater than 80.0% as determined by SDS-PAGE. |
| Description |
Recombinant Human PSIP1 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 61,546 Dalton. PSIP1 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques. |
| Protein Background |
PC4 and SFRS1 Interacting Protein 1 (PSIP1) is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. PSIP1 is also involved particularly in lens epithelial cell gene regulation and stress responses. PSIP1 has a significant role in lens epithelial to fiber cell terminal differentiation. In addition, PSIP1 plays a protective role during stress-induced apoptosis. Furthermore, PSIP1 is a cellular cofactor for lentiviral integration. |
| Expression host |
Sf9 insect cells. |
| Synonyms |
PC4 and SFRS1 Interacting Protein 1, Lens Epithelium-Derived Growth Factor, Transcriptional Coactivator P75/P52, Dense Fine Speckles 70 KDa Protein, CLL-Associated Antigen KW-7, DFS 70, PSIP2, DFS70, LEDGF, PC4 And SFRS1 Interacting Protein 2, Transcriptional Coactivator P52/P75, PAIP, P52, P75, PSIP1. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Immunological functions |
1. Binds IgG type human auto-antibodies.2. Immunodot and microarray test with positive/negative sera panels. |
