| Formulation |
PI3 protein solution (0.25mg/ml) containing 20mM Tris-HCl (pH8.0), 0.15M NaCl,10% glycerol and 1mM DTT. |
| Purity |
Greater than 80.0% as determined by SDS-PAGE. |
| Description |
PI3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 118 amino acids (23-117 a.a) and having a molecular mass of 12kDa.PI3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Peptidase Inhibitor 3 also known as PI3 is an elastase-specific protease inhibitor, which contains a WAP-type four-disulfide core (WFDC) domain, and therefore belongs to the WFDC domain family. The majority WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric & telomeric. PI3 belongs to the centromeric cluster. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Peptidase Inhibitor 3, Skin-Derived, Skin-Derived Antileukoproteinase, Protease Inhibitor 3, Skin-Derived (SKALP), WAP Four-Disulfide Core Domain Protein 14, Elastase-Specific Inhibitor, Protease Inhibitor WAP3, Trappin-2, WFDC14, SKALP, PI-3, WAP3, ESI, WAP Four-Disulfide Core Domain 14, Peptidase Inhibitor 3, Pre-Elafin, Cementoin, Elafin, PI3 . |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSAVTGVPV KGQDTVKGRV PFNGQDPVKG QVSVKGQDKV KAQEPVKGPV STKPGSCPII LIRCAMLNPP NRCLKDTDCP GIKKCCEGSC GMACFVPQ |
