| Reconstitution |
1mg/ml in PBS (after reconstitution)Shipping ConditionsAntibody is shipped lyophilized at ambient temperature. |
| Formulation |
Lipocalin-2 is supplied in PBS, 50% glycerol. |
| Solubility |
MCAF does not bind to plastic as good as most proteins - use higher concentration for proper binding. |
| Purity |
Greater than 95% as determined by SDS-PAGE. Single band on Western Blot. |
| Description |
Neutrophil Gelatinase Associated Lipocalin Human Recombinant is expressed in E. coli having a molecular weight of 28.1 kDa fused to an amino terminal hexahistidine tag.The LCN2 is purified by proprietary chromatographic techniques. |
| Protein Background |
Recombinant Human Lipocalin-2 also called Neutrophil Gelatinase Associated Lipocalin (NGAL) belongs to a family of lipocans which include 25 proteins (including a1-microglobulin and b-lactoglobulin), which are characterized by their ability to bind small lipophilic substances in their hydrophobic core.They thereby serve as transporters of substances like retinal, biliverdins & prostaglandins. There are indications that NGAL is involved in modulation of the inflammatory response and is found in the plasma of patients after stroke. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Neutrophil gelatinase-associated lipocalin, NGAL, p25, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, LCN2. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Storage Conditions |
Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Antibody Properties |
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Protein content |
In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:5,000 dilution will give 0.3 O.D within 10 minutes. |
