LCN2 Human, His

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution)Shipping ConditionsAntibody is shipped lyophilized at ambient temperature.

Formulation

Lipocalin-2 is supplied in PBS, 50% glycerol.

Solubility

MCAF does not bind to plastic as good as most proteins - use higher concentration for proper binding.

Purity

Greater than 95% as determined by SDS-PAGE. Single band on Western Blot.

Description

Neutrophil Gelatinase Associated Lipocalin Human Recombinant is expressed in E. coli having a molecular weight of 28.1 kDa fused to an amino terminal hexahistidine tag.The LCN2 is purified by proprietary chromatographic techniques.

Protein Background

Recombinant Human Lipocalin-2 also called Neutrophil Gelatinase Associated Lipocalin (NGAL) belongs to a family of lipocans which include 25 proteins (including a1-microglobulin and b-lactoglobulin), which are characterized by their ability to bind small

Expression host

Escherichia Coli.

Synonyms

Neutrophil gelatinase-associated lipocalin, NGAL, p25, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, LCN2.

Reagent Appearance

Sterile Filtered clear solution.

Storage Conditions

Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Antibody Properties

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Protein content

In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:5,000 dilution will give 0.3 O.D within 10 minutes.