| Formulation |
The HOMER3 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 150mM NaCl, 1mM DTT and 30% glycerol. |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Inactivation |
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-17 as a Reference Standard. |
| Description |
HOMER3 Human Recombinant produced in E. coli is a single polypeptide chain containing 386 amino acids (1-361) and having a molecular mass of 42.5kDa.HOMER3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
HOMER3 belongs to the HOMER family of postsynaptic density scaffolding proteins which have an analogous domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain that facilitates protein-protein relations, and two leucine zipper and a carboxy-terminal coiled-coil domain motifs which take part in self-oligomerization. |
| Expression host |
E.coli. |
| Synonyms |
Homer homolog 3 (Drosophila)1 2 , HOMER-31 2 , homer protein homolog 32 , Homer neuronal immediate early gene 32, Homer-33, VESL32. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSHMMSTAR EQPIFSTRAH VFQIDPATKR NWIPAGKHAL TVSYFYDATR NVYRIISIGG AKAIINSTVT PNMTFTKTSQ KFGQWADSRA NTVYGLGFAS EQHLTQFAEK FQEVKEAARL AREKSQDGGE LTSPALGLAS HQVPPSPLVS ANGPGEEKLF RSQSADAPGP TERERLKKML SEGSVGEVQW EAEFFALQDS NNKLAGALRE ANAAAAQWRQ QLEAQRAEAE RLRQRVAELE AQAASEVTPT GEKEGLGQGQ SLEQLEALVQ TKDQEIQTLK SQTGGPREAL EAAEREETQQ KVQDLETRNA ELEHQLRAME RSLEEARAER ERARAEVGRA AQLLDVSLFE LSELREGLAR LAEAAP |
