GM2AP1 Human

Data sheet

Formulation

GM2AP1 was filtered (0.4um) and lyophilized from 0.5mg/ml in 0.05M Tris buffer and 0.1M NaCl, pH 8.0.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. GM2AP1 is not sterile! Please filter the product by an appropriate sterile filter before using it in

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

GM2AP1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Ser33-Ile193) containing 170 amino acids including a 9 aa His tag at N-terminus. The total calculated molecular mass is 18.7kDa.

Protein Background

GM2 Ganglioside Activator Pseudogene 1 (GM2AP1) is a small glycolipid transport protein which functions as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. Beta-hexosaminidase A, along with GM2AP1, catalyzes the degradation o

Expression host

Escherichia Coli.

Synonyms

GM2 Ganglioside Activator, Cerebroside Sulfate Activator Protein, Sphingolipid Activator Protein 3, GM2-AP, SAP-3, GM2 Ganglioside Activator Protein, Shingolipid Activator Protein 3, GM2A.

Reagent Appearance

Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

MKHHHHHHAS FSWDNCDEGK DPAVIRSLTL EPDPIIVPGN VTLSVMGSTS VPLSSPLKVD LVLEKEVAGL WIKIPCTDYI GSCTFEHFCD VLDMLIPTGE PCPEPLRTYG LPCHCPFKEG TYSLPKSEFV VPDLELPSWL TTGNYRIESV LSSSGKRLGC IKIAASLKGI.