| Formulation |
Lyophilized from a concentrated (1mg/ml) solution containing 0.1% Trifluoroacetic Acid (TFA). |
| Solubility |
It is recommended to reconstitute the lyophilized GDF11 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity |
Greater than 97.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE. |
| Description |
GDF11 Human Recombinant produced in E.Coli is a non-glycosylated homodimer containing 2x109 amino acids and having a total molecular mass of 25.0kDa. |
| Protein Background |
GDF-11 belongs to the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. GDF-11 is a central developmental factor which controls muscular and neural development. In adults, GDF-11 encourages cardiac hypertrophy reverse by the revival of cardiomyocytes. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Growth Differentiation Factor 11, GDF-11, Bone Morphogenetic Protein 11, BMP11. |
| Reagent Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability |
Lyophilized GDF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF11 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence |
NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHLVQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS |
| Biological Activity |
The ED50 as determined by the ability to inhibit alkaline phosphatase activity in ATDC5 cells, is 0.2-0.3ng/ml, corresponding to a specific activity of 5 x 106units/mg. |
| Bilogical Activity |
BCA - to determine content of the protein. SDS PAGE - to determine purity of the protein. |
