GDF11 Human

Data sheet

Formulation

Lyophilized from a concentrated (1mg/ml) solution containing 0.1% Trifluoroacetic Acid (TFA).

Solubility

It is recommended to reconstitute the lyophilized GDF11 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.

Description

GDF11 Human Recombinant produced in E.Coli is a non-glycosylated homodimer containing 2x109 amino acids and having a total molecular mass of 25.0kDa.

Protein Background

GDF-11 belongs to the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. GDF-11 is a central developmental factor which controls muscular and neural development. In adults, GDF-11 encourages cardiac hypertrophy reverse by the revival of

Expression host

Escherichia Coli.

Synonyms

Growth Differentiation Factor 11, GDF-11, Bone Morphogenetic Protein 11, BMP11.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized GDF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF11 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a

Amino acid sequence

NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHLVQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS

Biological Activity

The ED50 as determined by the ability to inhibit alkaline phosphatase activity in ATDC5 cells, is 0.2-0.3ng/ml, corresponding to a specific activity of 5 x 106units/mg.

Bilogical Activity

BCA - to determine content of the protein. SDS PAGE - to determine purity of the protein.