ENO2 Antibody

Data sheet

Clone

PAT17D10A

Applications

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western bl

Protein Background

Neuron-specificenolase also caled NSE is a glycolytic isoenzyme which is situated in central and peripheral neurons and neuroendocrine cells. Enolase-2 is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine

Synonyms

Gamma-enolase, EC 4.2.1.11, 2-phospho-D-glycerate hydro-lyase, Neural enolase, Neuron-specific enolase, NSE, Enolase 2, ENO2.

Reagent Appearance

Sterile filtered colorless solution.

Type

Mouse Anti Human Monoclonal.

Immunogen

Anti-human ENO2 mAb, clone PAT17D10A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ENO2 protein 1-434 amino acids purified from E. coli.

Ig Subclass

Mouse IgG2b heavy chain and Kappa light chain.

Purification Method

ENO2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.

Protein formulation

1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.

Storage Procedures

For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.

Stability / Shelf Life

12 months at -20°C. 1 month at 4°C.