Data sheet
| Assay Range | 78.125 - 5000 pg/ml |
More info
Sensitivity
70 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human TIE1 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human TIE1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human TIE1 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
TIE1 is a cell surface protein expressed exclusively in endothelial cells, however it has also been shown to be expressed in immature hematopoietic cells. TIE1 upregulates the cell adhesion molecules (CAMs) VCAM-1, E-selectin, and ICAM-1 through a p38-dependent mechanism. Attachment of monocyte derived immune cells to endothelial cells is also enhanced by TIE1 expression. TIE1 has a proinflammatory effect and may play a role in the endothelial inflammatory diseases such as atherosclerosis.
