Data sheet
| Assay Range | 39 - 2500 pg/ml |
More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human COMP standard: 2 vials.
2. One 96-well plate precoated with anti- Human COMP Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Human Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5 (TSP-5), is a member of the Thrombospondin (TSP) protein family which includes subgroup A (TSP-1 and TSP-2) and subgroup B (TSP-3, TSP-4 and TSP-5/COMP). COMP is composed of a non-collagenous coiled-coil domain, four EGF-like repeats, seven TSP type 3 repeats, and a globular TSP C-terminal domain. The coiled-coil domain mediates the association of COMP into disulfide-linked homopentamers. COMP is predominantly found in the extracellular matrix of cartilage, tendons, and ligaments. It plays a pivotal role in endochondral ossification and in the assembly and stabilization of the extracellular matrix. COMP maintains the structural integrity of the cartilage through its interaction with a number of extracellular matrix proteins. COMP can bind to collagens type I and II via its C-terminal globular domain and act as a catalyst to promote fibril formation. COMP can inhibit cell proliferation while enhancing chondrogenesis. COMP can also protect chondrocytes from cell death by increasing production of survival proteins. It is reported that COMP is subjected to proteolytic cleavage in the cartilage of Arthritis’ patients. These fragments are detectable in the circulation and may be used as diagnostic and prognostic indicators and biomarkers for disease severity and response to treatment.